ibright analysis software (Thermo Fisher)
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ibright analysis software
Ibright Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ibright+analysis+software/ibright+analysis+software/pmc12271458-168-6-9
Average 90 stars, based on 1 article reviews
Ibright Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ibright+analysis+software/ibright+analysis+software/pmc12271458-168-6-9
Average 90 stars, based on 1 article reviews
ibright analysis software - by Bioz Stars,
2026-11
90/100 stars
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Software:Article Title: Chronic haloperidol exposure impairs neurodevelopment via Notch1 signaling in human stem cell-derived brain organoids Article Snippet: Detection of specific proteins was carried out with an ECL Western blotting kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Signal intensity was analyzed using the Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Article Title: Nostoc commune-derived scytonemin induced mitochondrial cell death in leukemia models Article Snippet: Obtained data were normalized to total protein levels [obtained using No-StainTM Protein Labeling Reagent (Thermo Scientific, Rockford, IL, USA)]. .. Normalization and quantification of protein expression were performed using Article Title: Antiproliferative and pro-apoptotic effects of Pseudevernia furfuracea (L.) Zopf extract and its active component physodic acid via oxidative stress and DNA damage in breast cancer cells Article Snippet: Protein expression was normalised to total protein levels using No-StainTM Protein Labelling Reagent (Thermo Scientific, Rockford, IL, USA). .. The Article Title: The characterization of the biological effect of hypoxia-mimetic condition on angiogenic potential in mesenchymal stem cells derived from different origins. Article Snippet: Then, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology) at room temperature for 1 h. SuperSignal West Pico Plus Chemiluminescent Substrate (Thermo Fisher Scientific) was used to detect the specific protein blots, and chemiluminescent images were acquired using the iBright imaging system (Thermo Fisher Scientific). .. Specific protein intensities were quantified using the Article Title: Identification of a ferroptosis related genes signature and GDF15 contributing to a new perspective for the diagnosis of CRPC. Article Snippet: The total proteins were separated by SDS–PAGE and detected with the primary antibodies against GDF15 (Proteintech, #27455-1-AP). β-actin (Proteintech, #20536-1-AP) and β-tubulin (Proteintech, #10094-1-AP) were used as internal control. .. The blots were cut prior to hybridization with antibodies during blotting. other:Article Title: Impact of Nanoparticle Stiffness on Endosomal Escape and Signaling Pathways in Cytosolic Delivery. Article Snippet: To prevent Article Title: Multi-omics analysis uncovers differential growth kinetics of Japanese Encephalitis Virus in human and porcine macrophages Article Snippet: The PCA and volcano plot were generated using FactoMineR and ggplot2 packages, respectively, in RStudio. Control:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Lysis:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Molecular Weight:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Marker:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Electrotransfer:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Membrane:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Imaging:Article Title: Analysis of Autophagy Under Abiotic Stress in Arabidopsis Seedlings Expressing the GFP‐ATG8 Autophagosome Marker Article Snippet: .. Treated and control A. thaliana seedlings (see Basic Protocols and ) Liquid nitrogen 2× Laemmli sample buffer (see recipe) RIPA lysis buffer (see recipe) Protein assay (e.g., Pierce 660 nm Protein Assay, Thermo Fisher Scientific, cat. no. PI22660) 10%‐12% SDS‐PAGE gels Protein molecular weight marker Protein running buffer (see recipe) Protein transfer buffer (see recipe) Double‐distilled water (ddH O) Ponceau S solution (Boston BioProducts, cat. no. ST‐180) PBST (see recipe) 5% and 1% (w/v) non‐fat dry milk (Blotto, Santa Cruz Biotechnology, cat. no. sc‐2324) in PBST Anti‐GFP primary antibody (e.g., Sigma‐Aldrich, cat. no. 11814460001) Secondary antibody (e.g., HRP‐conjugated goat anti‐mouse IgG (H+L), Thermo Fisher Scientific, cat. no. A16066) Enhanced chemiluminescent substrate (e.g., SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, cat. no. PI34577) 1.5‐ and 2‐ml microcentrifuge tubes Micropestle (e.g., IBI Scientific, cat. no. IB47315) SDS‐PAGE apparatus (Bio‐Rad) Wet or semi‐dry electrotransfer apparatus (Bio‐Rad) Nitrocellulose or polyvinylidene difluoride (PVDF) membrane (Amersham Hybond) Chemiluminescent imaging system (e.g., Thermo Fisher Scientific iBright 1500 or Bio‐Rad ChemiDoc Imaging System) Image analysis software such as ImageJ (Schindelin et al., ; Schneider et al., ), Image Lab Software Suite (Bio‐Rad), or Expressing:Article Title: Nostoc commune-derived scytonemin induced mitochondrial cell death in leukemia models Article Snippet: Obtained data were normalized to total protein levels [obtained using No-StainTM Protein Labeling Reagent (Thermo Scientific, Rockford, IL, USA)]. .. Normalization and quantification of protein expression were performed using Article Title: Antiproliferative and pro-apoptotic effects of Pseudevernia furfuracea (L.) Zopf extract and its active component physodic acid via oxidative stress and DNA damage in breast cancer cells Article Snippet: Protein expression was normalised to total protein levels using No-StainTM Protein Labelling Reagent (Thermo Scientific, Rockford, IL, USA). .. The Hybridization:Article Title: Identification of a ferroptosis related genes signature and GDF15 contributing to a new perspective for the diagnosis of CRPC. Article Snippet: The total proteins were separated by SDS–PAGE and detected with the primary antibodies against GDF15 (Proteintech, #27455-1-AP). β-actin (Proteintech, #20536-1-AP) and β-tubulin (Proteintech, #10094-1-AP) were used as internal control. .. The blots were cut prior to hybridization with antibodies during blotting. |